TABLE OF CONTENT
Tile Page i
Approval Page ii
Dedication iii
Acknowledgment iv
Table of Content v
List of Figures
List of Table
Abstract
CHAPTER ONE
1.0 Introduction 1
1.1 Problem of Statement 13
1.2 Justification 14
1.3 Aim and Objective of the study 15
CHAPTER TWO
2.0 Literature Review 17
CHAPTER THREE
3.0 Materials and Method 29
3.1 Sterilization of Materials 29
3.2 Sample Size 29
3.3 Collection and Processing Sample 30
3.4 Culture Media 30
3.5 Isolation of Cultures 31
3.6 Characterization of Bacteria Isolates 32
3.7 Colonial Morphology 32
3.8 Cellular Morphology 32
3.9 Biochemical Characterization 34
3.10 Antibiotics Sensitivity Test 36
CHAPTER FOUR
4.0 Results 39
CHAPTER FIVE
5.0 Discussion 60
5.1 Conclusion 66
5.2 Recommendation 67
References
LIST OF FIGURES
Figure 1: Frequency of Occurrence of bacterial from Wound Infection patient in University of Ilorin Teaching Hospital.
Figure 2: Bacterial Isolate from patient with diabetic ulcers in University of Ilorin Teaching Hospital.
Figure 3: Bacterial Isolated from patients with surgical wound in University of Ilorin Teaching Hospital.
Figure 4: Bacterial Isolated from patients with burns in University of Ilorin Teaching Hospital.
Figure 5: bacterial Isolated from patient with pressure sore in University of Ilorin Teaching Hospital.
LIST OF TABLE
Table 1: Morphological Characteristics, Staining Reaction and Biochemical Test
Table 2: Frequency of Occurrence of bacteria from Wound Infection patient in University of Ilorin Teaching Hospital.
Table 3: Bacteria pathogens causing wound infection in patients with Diabetic ulcers.
Table 4: Bacteria pathogens causing wound infection in patients with Surgical Wound.
Table 5: Bacteria pathogens causing wound infection in patients with Burns.
Table 6: Bacteria pathogens causing wound infection in patients with Pressure Sore.
Table 7: Antibiotic susceptibility Patterns of Staphylococcus aureus isolated from wound patients in UITH
Table 8: Antibiotics susceptibility pattern of Pseudomonas aeruginosa isolated from wound in UITH
Table 9: Antibiotic susceptibility pattern of Staphylococcus epidermidis in UITH.
Table 10: Antibiotic Susceptibility pattern of Escherichia coli isolated from wound patients in UITH.
Table 11: Antibiotic Susceptibility pattern of Streptococcus pyogenes isolated from wound patients in UITH.
Table 12: Antibiotic Susceptibility pattern of Klebsiella pneumoniae isolated from wound patients in UITH.
ABSTRACT
This work investigated the antibiotic susceptibility profile of bacterial associated with wound sepsis of patients attending University of Ilorin, Ilorin, Teaching Hospital. Wound swabs were collected from a total number of Hundred patients with different kinds of wound (surgical wound, burn, ulcers and pressure sores) and cultured, of which 72 samples showed bacterial growth. Six different species of bacteria were isolated. Staphylocococcus aureus (47.2%) Pseudomonas aeruginosa (19.5%), Klebsiella pneumonia (6.85%), Escherichia coli (15.3%), Staphylococcus epidemidis (5.6%) and Streptococcus pyogenes (5.6%). The antibiotics susceptibility test of these bacterial isolate was performed using the Kirby- bauer dist diffusion method. Gantamycin and Sreptomycin shows high effectiveness to all the isolate except Staphylococcus epidermis and Klebsiella with pefloxacin and ceftadine respectively showing sensitivity to them. Resistance were shown amongst Ampicillin, contrimoxazole and Tetracycline. This study has revealed Gentamycin as the only antibiotic to which most bacterial isolate from infected wounds were sensitive to. Ampicillin and penicillin were effective against Streptococcus pygenes while zinacef and ceftazidime shows high effectiveness against Klebsiella pneumonia.
KEYWORDS: wound infection: antibiotics susceptibility profile: staphylococcus aureus: gentamycin.
